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wild type m pneumoniae strain m129 b7  (ATCC)


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    Structured Review

    ATCC wild type m pneumoniae strain m129 b7
    Wild Type M Pneumoniae Strain M129 B7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+pneumoniae+m129+b7/B-7/bio_rxiv__64898__2026__04__12__717927-147-15-20
    Average 96 stars, based on 527 article reviews
    wild type m pneumoniae strain m129 b7 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Detection of Mycoplasma pneumoniae and Legionella pneumophila in Patients Having Community-Acquired Pneumonia: A Multicentric Study from New Delhi, India
    Article Snippet: .. The 543 bp PCR product from M. pneumoniae M129- B7 and 375 bp PCR product from L. pneumophila ATCC 33153 standard strains were cloned in pGEM-T Easy (Promega, Madison, WI) vector according to manufactures instructions. ..

    Clone Assay:

    Article Title: Detection of Mycoplasma pneumoniae and Legionella pneumophila in Patients Having Community-Acquired Pneumonia: A Multicentric Study from New Delhi, India
    Article Snippet: .. The 543 bp PCR product from M. pneumoniae M129- B7 and 375 bp PCR product from L. pneumophila ATCC 33153 standard strains were cloned in pGEM-T Easy (Promega, Madison, WI) vector according to manufactures instructions. ..

    Plasmid Preparation:

    Article Title: Detection of Mycoplasma pneumoniae and Legionella pneumophila in Patients Having Community-Acquired Pneumonia: A Multicentric Study from New Delhi, India
    Article Snippet: .. The 543 bp PCR product from M. pneumoniae M129- B7 and 375 bp PCR product from L. pneumophila ATCC 33153 standard strains were cloned in pGEM-T Easy (Promega, Madison, WI) vector according to manufactures instructions. ..

    Selection:

    Article Title: In Silico Design and Characterization of a Multi-Epitope Vaccine Candidate Against Mycoplasma pneumoniae Using a Reverse Vaccinology Strategy
    Article Snippet: .. First, in terms of antigen selection, Mahmood et al. (2021) [42] and Shahbazi et al. (2025) [26] selected highly antigenic proteins from single strains, such as M. pneumoniae ATCC 29342/M129 and M. pneumoniae M129-B7; however, in this study, we chose four important structural proteins (HMW1–3, p1-adhesive protein) from different strains of M. pneumoniae as antigens and generated conserved consensus sequences respectively to ensure a broad antigen coverage. ..

    Article Title: In Silico Design and Characterization of a Multi-Epitope Vaccine Candidate Against Mycoplasma pneumoniae Using a Reverse Vaccinology Strategy
    Article Snippet: .. First, in terms of antigen selection, Mahmood et al. (2021) [ ] and Shahbazi et al. (2025) [ ] selected highly antigenic proteins from single strains, such as M. pneumoniae ATCC 29342/M129 and M. pneumoniae M129-B7; however, in this study, we chose four important structural proteins (HMW1–3, p1-adhesive protein) from different strains of M. pneumoniae as antigens and generated conserved consensus sequences respectively to ensure a broad antigen coverage. ..

    Generated:

    Article Title: In Silico Design and Characterization of a Multi-Epitope Vaccine Candidate Against Mycoplasma pneumoniae Using a Reverse Vaccinology Strategy
    Article Snippet: .. First, in terms of antigen selection, Mahmood et al. (2021) [42] and Shahbazi et al. (2025) [26] selected highly antigenic proteins from single strains, such as M. pneumoniae ATCC 29342/M129 and M. pneumoniae M129-B7; however, in this study, we chose four important structural proteins (HMW1–3, p1-adhesive protein) from different strains of M. pneumoniae as antigens and generated conserved consensus sequences respectively to ensure a broad antigen coverage. ..

    Article Title: In Silico Design and Characterization of a Multi-Epitope Vaccine Candidate Against Mycoplasma pneumoniae Using a Reverse Vaccinology Strategy
    Article Snippet: .. First, in terms of antigen selection, Mahmood et al. (2021) [ ] and Shahbazi et al. (2025) [ ] selected highly antigenic proteins from single strains, such as M. pneumoniae ATCC 29342/M129 and M. pneumoniae M129-B7; however, in this study, we chose four important structural proteins (HMW1–3, p1-adhesive protein) from different strains of M. pneumoniae as antigens and generated conserved consensus sequences respectively to ensure a broad antigen coverage. ..



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    Sensitivity of the LAMP reaction for the detection of S. <t>pneumoniae</t> , S. aureus , H. influenzae , and M. pneumoniae. Visual LoD determined for the detection of S. pneumoniae (3.9 ×10 3 CFU/mL), S. aureus (1.7 ×10 5 CFU/mL), H. influenzae (8.2 ×10 3 CFU/mL), and M. pneumoniae (1.27 ×10 3 genome copies/reaction) (A) . Verification of amplification by 2% agarose gel electrophoresis (B) .
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    Image Search Results


    Sensitivity of the LAMP reaction for the detection of S. pneumoniae , S. aureus , H. influenzae , and M. pneumoniae. Visual LoD determined for the detection of S. pneumoniae (3.9 ×10 3 CFU/mL), S. aureus (1.7 ×10 5 CFU/mL), H. influenzae (8.2 ×10 3 CFU/mL), and M. pneumoniae (1.27 ×10 3 genome copies/reaction) (A) . Verification of amplification by 2% agarose gel electrophoresis (B) .

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

    doi: 10.3389/fmicb.2026.1748456

    Figure Lengend Snippet: Sensitivity of the LAMP reaction for the detection of S. pneumoniae , S. aureus , H. influenzae , and M. pneumoniae. Visual LoD determined for the detection of S. pneumoniae (3.9 ×10 3 CFU/mL), S. aureus (1.7 ×10 5 CFU/mL), H. influenzae (8.2 ×10 3 CFU/mL), and M. pneumoniae (1.27 ×10 3 genome copies/reaction) (A) . Verification of amplification by 2% agarose gel electrophoresis (B) .

    Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

    Techniques: Amplification, Agarose Gel Electrophoresis

    Sensitivity of the designed primers for the detection of K. pneumoniae. Visual LoD (A) . The calculated LoD was 1.5 ×10 4 CFU/mL. Amplification was confirmed by 2% agarose gel electrophoresis (B) .

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

    doi: 10.3389/fmicb.2026.1748456

    Figure Lengend Snippet: Sensitivity of the designed primers for the detection of K. pneumoniae. Visual LoD (A) . The calculated LoD was 1.5 ×10 4 CFU/mL. Amplification was confirmed by 2% agarose gel electrophoresis (B) .

    Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

    Techniques: Amplification, Agarose Gel Electrophoresis

    Relationship between time to positivity and bacterial load for LAMP detection of S. pneumoniae , S. aureus , and H. influenzae . As the bacterial concentration decreased, the time required for detection increased across all three pathogens. For S. pneumoniae , high concentrations produced a clear positive result within 35 min. However, concentrations near the LoD (10 4 and 10 3 CFU/mL) required more than 55 min to be considered positive. Similarly, S. aureus showed a positive signal at 35 min when tested at high concentrations, whereas lower concentrations (around 10 4 CFU/mL) required over 45. In the case of H. influenzae , the highest concentration yielded a visible positive reaction at 25 min, while lower concentrations needed up to 45 min. Transition phase: In all cases, there was a stage where tubes began to show a greenish signal, indicating the onset of positivity, although the color had not yet fully developed to a strong green signal.

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia

    doi: 10.3389/fmicb.2026.1748456

    Figure Lengend Snippet: Relationship between time to positivity and bacterial load for LAMP detection of S. pneumoniae , S. aureus , and H. influenzae . As the bacterial concentration decreased, the time required for detection increased across all three pathogens. For S. pneumoniae , high concentrations produced a clear positive result within 35 min. However, concentrations near the LoD (10 4 and 10 3 CFU/mL) required more than 55 min to be considered positive. Similarly, S. aureus showed a positive signal at 35 min when tested at high concentrations, whereas lower concentrations (around 10 4 CFU/mL) required over 45. In the case of H. influenzae , the highest concentration yielded a visible positive reaction at 25 min, while lower concentrations needed up to 45 min. Transition phase: In all cases, there was a stage where tubes began to show a greenish signal, indicating the onset of positivity, although the color had not yet fully developed to a strong green signal.

    Article Snippet: Initially, tests included panel bacteria: S. pneumoniae ATCC 49619, S. aureus ATCC 25923, H. influenzae ATCC 49766, and purified DNA from M. pneumoniae (ATCC 29342DQ).

    Techniques: Concentration Assay, Produced